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Portable AKVIS Sketch v14.0.2545. All Files Right Here!Characteristics of the membrane electric potential and calcium current in proximal tubules of the rabbit.
The micro-electrode recording method was applied to the proximal tubules of the rabbit in order to study the membrane properties of the cells. It was found that the cell membrane potential was significantly higher than the resting potential. Only in the absence of bath CaCl2, the presence of low concentrations of CaCl2 (0.1-1.0 mM) caused hyperpolarization. In cells bathed in CaCl2-containing solution (1.2 mM), exposure to both nifedipine (0.1-100 microM) and cadmium (0.1-1.0 mM) depolarized the cells, and the application of the two drugs could antagonize each other’s action. The time constant of the activation of the current, as well as the slope of the current-voltage (I-V) relation, decreased with increasing CaCl2. The current could be divided into two components: (1) a rapidly activating and inactivating component, and (2) a slowly activating component with a small slope of the I-V relation. The latter component was due to the Ca2(+)-dependent slow Ca2(+)-activated K+ (SK-Ca) current. The role of SK-Ca current and the mechanisms underlying the cell membrane potential were discussed.THE SEXIEST IN THE WILDLIFE
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